Welcome to the Rossi Biophotonics Lab website! Dr. Vince Rossi is the PI of the lab, bringing Biophotonics research to the Department of Physics & Astronomy at Washburn University. The Biophotonics Lab started in the Fall of 2018, so just as the lab is building infrastructure and developing research projects, the website will also be under continued development. Please check back for more information!
Are you interested in Biophotonics research? The Biophotonics Lab is looking for undergraduate researchers—you needn't be a physics major. If you're interested in doing research in the lab, contact Dr. Rossi.
The Biophotonics Lab offers a broad range of projects for undergraduate research. Physics operates at the intersection between rational and empirical knowledge—between theory and experiment. While the Biophotonics Lab is in principle an experimental lab, offering students experience in optical design, the experimental work we do here is generally supported by computational modeling. Beyond optical design and computational modeling, students have the opportunity to learn computer interfacing, experimental design, image processing and analysis.
Digital holographic Microscopy (DHM) offers label-free, real-time imaging of cells and other biological samples as a means of monitoring their response to experimental conditions. Moreover, since holography is a means of recovering the whole electric field (amplitude & phase) from the sample, DHM allows us to generate a 3-D reconstruction of the sample via a single holographic image.
The phase of an electrodynaimc wave (light) changes upon interaction with a sample due to differences in the local depth and index of refraction of the sample (as compared to its surroundings). Quantitative Phase Imaging (QPI) is a means of measuring the resulting phase of light after interacting with a sample. Given the initial phase, the detected phase can be quantitatively related back to the sample characteristics.
Light scattered from intracellular objects such as mitochondria and nuclei have distinctive scattering functions. By imaging the scattered light, we are thereby able to determine information about the scatterers themselves. We use Optical Scatter Imaging (OSI) in order to gain information about intracellular structures and how their morphologies react to different stimuli.
The Biophotonics Lab will be working in conjunction with Dr. Jason Emry (Biology) to image microbes in botanical samples via fluorescence microscopy. More to follow....
Photodynamic Therapy (PDT) is a targeted therapy for treating cancers and infections caused by antibiotic resistant bacteria. A photosensitizer, light and molecular oxygen are required for PDT. Light excites the photosensitizer upon absorption. Once in an excited energy level, the photosensitizer can transfer energy to ambient molecular oxygen via electron transfer, thereby creating reactive oxygen species that damage their immediate surroundings.